Abstract
Electrophoresis of serum proteins is one of the traditional applications of zone electrophoresis. Whereas electrophoresis in supporting media uses usually 5,5′-diethylbarbiturate at pH 8.6 as the buffer, in capillary zone electrophoresis with on-line UV detection, this electrolyte is of little use because of its high UV absorbance. For that reason, a number of operational electrolytes differing in composition were tested for use in capillary electrophoresis of serum proteins. The influence of the p K A of co-ions and counter ionsand the concentration of the operational electrolyte was examined. If 0.1 M methylglucamine-0.1 M ϵ-aminocaproic acid or 0.1 M methylglucamine, −0.1 γ-aminobutyric acid is used as the operational electrolyte, capillary electrophoresis separates serum proteins into more than ten zones.
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