Abstract
Methods for the determination of non-UV-absorbing charged copolymers and polyelectrolytes by capillary electrophoresis with indirect UV detection (IUV) and contactless conductivity detection (CCD) were developed. Synthetic poly(ethylene glycol)-poly(methacrylic acid) copolymers (PEG-MAA) with different charge densities were separated in an arginine/sorbate buffer by free solution capillary electrophoresis. Blends of PEG-MAA copolymers and a high molar mass poly(methacrylic acid) sodium salt standard PMAA75k (M w = 75 kg/mol) have been separated, and the effect of the charge density on the detector signals has been investigated. The new experimental protocol enables the simultaneous recording of the CCD and the IUV signal in one single run. The arginine/sorbate buffer provides sufficient signal-to-noise ratios for indirect UV detection and contactless conductivity detection.
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