Capillary electrophoresis detection scheme for vitamin B1 and vitamin B2 based on the potassium hexacyanoferrate(III) chemiluminescence system

Abstract

A novel chemiluminescence (CL) detection scheme for the simultaneous determination of vitamin B1 and vitamin B2 has been developed. After being separated by capillary electrophoresis (CE), the analytes were determined by direct CL of vitamin B1 and vitamin B2-potassium hexacyanoferrate(III) (K3[Fe(CN)6]) in alkaline aqueous solution. Parameters affecting CE-CL separation and detection, such as the pH value and the concentration of electrolyte and K3[Fe(CN)6] were optimized. Vitamin B1 and vitamin B2 are completely resolved with migration times shorter than 5 min. Under the optimal conditions, a linear range from 0.04 to 50, 0.08 to 90 µg mL−1 and a detection limit of 0.01, 0.03 µg mL−1 (signal-to-noise ratio = 3) for vitamin B1 and vitamin B2 respectively, were achieved. The relative standard deviations (R.S.D.) for 0.5 µg mL−1 of vitamin B1 and 0.8 µg mL−1 of vitamin B2 (n = 11) were 2.9 and 3.7%, respectively. The applicability of the method was examined for the analysis of pharmaceutical tablets.