Capillary density and succinate dehydrogenase in skeletal muscle on chronic kidney disease rats

Abstract

Background and PurposeKidney disease increases risk of blood vessel and capillary disease. In case of chronic kidney disease (CKD), these problems may happen slowly over a long period of time. The thing about heart in particular is well known. Chronic kidney disease may be caused by diabetes, high blood pressure and other disorders. Hypertension causes CKD and CKD causes hypertension. Anyone can get CKD at any age and any sex. However, some people are more easily to get kidney disease than others. That two main causes of CKD are diabetes and high blood pressure. Those diseases fail with blood vessel and capillary in various factors. In any case, the blood vessel system is connected with kidney disease deeply. We confirmed muscle capillary architecture on rat last time. We clarified muscle capillary density and mitochondrial activity this time.Materials/Methods12 Wistar rats (age; 16wks) were randomly divided into CKD group (n=6) and Sham operation (SHAM) group (n=6). Rats in CKD group suffered from a chronic kidney failure artificially. The details are as follows; 1. When 16wks old, under anesthesia with pentobarbital, we removed the two‐third left kidney surgically. 2. When 17wks old, under anesthesia with pentobarbital, we removed the right kidney surgically. 3. When 23wks old, we removed soleus muscle out. 4. Those muscles were frozen, were stained by SDH, AP and ATPase (pH4.2) method, and observed with light microscope (LM). In SHAM group, they were operated for at the same time, but the kidney was not removed surgically. The kidney was extracted to outside from rat and it was returned to rat without removing it surgically. The microscope image of SDH stained was analyzed in NIH Image software and it was compared. All data were presented as the means ± SEM. All statistical tests were done using an unpaired Student's – test. P<0.05 indicates a significant difference.ResultsOn 23 wks old CKD, body weight and soleus weight were decrease. Feeding volumes were same. Urinary albumin/urinary creatine value was increase. Data from measurement of the LM images by the NIH Image software of SDH, the mean SDH activity on CKD rats were significantly higher than that in the SHAM rats. Capillary density on CKD rat was lower than that in SHAM. However, muscle myosin heavy chain types of ratio on CKD were same to SHAM.ConclusionsThese results indicate that muscle requires oxygen, but shows that blood supply to muscle decreases on CKD.Support or Funding InformationThis study was supported by Grants‐in‐Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (Grant no. 17K01879)This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.