Capillary architecture in skeletal muscle on chronic kidney disease rats

Abstract

Background and PurposeKidney disease increases risk of blood vessel and capillary disease. In case of chronic kidney disease (CKD), these problems may happen slowly over a long period of time. The thing about heart in particular is well known. Chronic kidney disease may be caused by diabetes, high blood pressure and other disorders. Hypertension causes CKD and CKD causes hypertension. Anyone can get CKD at any age and any sex. However, some people are more easily to get kidney disease than others. That two main causes of CKD are diabetes and high blood pressure. Those diseases fail with blood vessel and capillary in various factors. In any case, the blood vessel system is connected with kidney disease deeply. However, muscle capillary architecture of kidney disease is not observed. Our aim is to make skeletal muscle capillary architecture in CKD clear.Materials/Methods12 Wistar rats (age; 16wks) were randomly divided into CKD group (n=6) and Sham operation (SHAM) group (n=6). Rats in CKD group suffered from a chronic kidney failure artificially. The details are as follows; 1. When 16wks old, under anesthesia with pentobarbital, we removed the right kidney surgically. 2. When 17wks old, under anesthesia with pentobarbital, we removed the two‐third left kidney surgically. 3. When 23wks old, we injected fluorescer into soleus muscle and removed it out. 4. Those muscles were frozen and were observed with confocal laser microscope. In SHAM group, they were operated for at the same time, but the kidney was not removed surgically. The kidney was extracted to outside from rat and it was returned to rat without removing it surgically. The microscope image was analyzed in NIH Image software and it was compared. All data were presented as the means ± SEM. All statistical tests were done using an unpaired Student's –test. P<0.05 indicates a significant difference.ResultsOn 23 wks old CKD, body weight and soleus weight were decrease. Feeding volumes were same. Urinary albumin/urinary creatine value was increase. Data from the morphological measurement of the CLSM images by the NIH Image software. The mean microvessel volume of CKD rats were significantly lower than that in the SHAM rats. Capillary architecture of CKD was looser than that in SHAM. Moreover, capillary diameter of CKD was smaller than that in SHAM.ConclusionsThese results indicate that capillary volume in skeletal muscle may decrease by only kidney disease. It not include diabetes and/or hypertension. Diabetes and/or hypertension also decrease capillary volume. These relations are our future issues.Support or Funding InformationThis study was supported by Grants‐in‐Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (Grant no. 10582190)