Abstract
Coproantibodies [1] in experimental cholera have been investigated to assess the effectiveness of oral vaccination [1-3], but the levels of intestinal antibody have been difficult to measure and to correlate with protective effects of the cholera vaccine. Recently, the canine species has been used as an animal model for experimental cholera because the syndrome of fluid and electrolyte loss can be produced [4]. Also, the lack of demonstrable gastrointestinal histopathology in this canine model [5] makes further analogy with the human illness possible. Since the dog is a useful experimental model and is subject to many of the diseases that occur in man, we have been interested in the canine immunological system, particularly in describing the immunoglobulins in serum [6, 7] and in external secretions [8, 9]. This paper will include a discussion of the immunoglobulin content of normal canine intestinal secretions and will quantitate the concentrations of these copro-immunoglobulins. An isolation procedure for recovery of the copro-immunoglobulins, particularly secretory IgA, will be outlined; in addition, some of the physical properties of the IgA molecule and its polypeptide-chain components will be examined.
Published Version
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