Candidate Quarantine Aerosols for Stored-Product Insects, 1980

Abstract

Abstract Laboratory-reared stored-product insects were exposed to insecticidol formulations in transport trailers (ca. 71 m3) in Miami, FL and Baltimore, MD. For each test, 40 insects of each species were held in 10-cm diam open petri dishes placed on the floor at the midsection of each trailer ca. 30 cm from a side wall. Freon-propelled aerosols were released within closed trailers, either from the center (total release) or by an individual walking the length of the trailer and spraying for the required amount of time. The exposure period was 5 hr; 10 min in the closed trailer after each aerosol application, 20 min with one door of the trailer opened for aeration, and 4.5 hr in the original containers after their removal from the trailers and before the insects were transferred to clean petri dishes. Insects were observed after 24 h for knockdown (those that could not walk or remain in an upright position) and at 14 days post-exposure for mortality, expressed as dead plus moribund insects (those that showed little or no movement when lightly stimulated with a small camel-hair brush). Each test was replicated at least 3 times. The average air temperature inside the trailer at treatment was 21° ± 3°C and 81 ± 4%RH.