Abstract

Abstract Laboratory-reared stored-product insects were exposed to insecticidal formulations in transport trailers (ca 32m or 71m) in Baltimore, MD and Miami, FL. For each test, 40 insects of each species were held in 10-cm diameter open petri dishes placed on the floor at the midsection of each trailer ca 30 cm from a side wall. The aerosols were propelled by freon 11/12 and released within closed trailers by an individual walking the length of the trailer and releasing the aerosol from the pressurized can for the required amount of time. Dusts were formulated with HiSil 233 as a carrier and introduced into the trailer by C0? propellant through an open rear door. The exposure period was 5 h; 10 min in the closed trailer after each aerosol or dust appnication, 20 min with one door of the trailer opened for aeration, and 4.5 h in the original containers after their removal from the trailers and before the insects (treated and untreated) were transferred to clean petri dishes. Insects were observed after 24 h for knockdown (KD) (those that could not walk or remain in an upright position) and at 14 days post-exposure for mortality, expressed as dead plus moribund (D+M) insects (those that showed little or no movement when lightly stimulated with a small camel-hair brush). Each test was replicated 3 times. The average air temperature inside the trailer at treatment was 25 ± 5 C.

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