Candida türlerinin flukonazol, vorikonazol, amfoterisin B'ye karşı duyarlılıklarının disk difüzyon ve mikrodilüsyon yöntemiyle araştırılması

Abstract

In this study, we aimed to investigate the antifungal susceptibility profile of Candida isolates obtained in our hospital with two methods. A total of 200 Candida isolates were obtained from samples of patients in various departments of our hospital. Identification of each strain was performed using conventional (germ tube formation, microscopic morphology in corn meal-Tween 80 agar), and commercial kit API 20C (Biomerieux, France). MIC values of fluconazole (FLU), voriconazole (VORI), and amphotericin B (AMB) were evaluated according to Clinical Laboratory Standards Institute broth microdilution and disk diffusion (DD) method. Totally isolated 200 Candida species were identified as 100 C. albicans, 25 C. glabrata, 7 C. krusei, 43 C. parapsilosis, and 25 C. tropicalis. All isolates were susceptible to VORI. High resistance rate (22,5%) was detected for AMB antifungal by reference microdilution method. Between two methods, the best categorical ratio was calculated as VORI (99%), FLU (84.9%), and AMB (72,5%) respectively. Voriconazole exhibited better activity in vitro than fluconazole, even in isolates fluconazole resistant and disk diffusion method can be considered as alternative antifungal sensitivity method for VORI.