Candida albicans の集落解離に関する研究 : I Sabouraud glucose agar 上の smooth→rough 解離

Abstract

The species of the pathogneic fungi have been diagnosed only on the basis of morphological aspect, especially of their colonial forms. The study of dissociation and variation is necessary to identify and classify the pathogenic fungi, according to one species has several morphological forms. The author have studied the causing of dissociation of the Rough form of colonies (so called "membranous variants" with Mackinon) that happened the appearance of variants on Sabouraud's glucose agar in Candida albicans. I have fixed two types of variants so called Smooth and Rough forms of colonies with one strain of Candida albicans and had comparative investigations of cultural, fermetatvie and antigenic properties between those two types of variants. The results of experiments are summarized as follows : 1. The great causing to arise dissociation of the S-R form are lack of carbohydrate, contained in the medium. And due to the higher pH of culture medium (from pH 7.2 to the Alkaline side), and it rapidly tended to dissociate by heating the culture medium in the same range of pH. 2. Morphological properties. -The S form on Sabouraud's glucose agar have not tendency toward R form in the young culture but it became intermediate form between the S and R form in the adult culture. The R form variants are sufficiently fixed to be considered as true variants. 3. Biochemical properties. -The R form variant ferments the same sugars as the S form. 4. In antigenic properties, the R form variants are observed with decrease of somatic polysaccharide-containing antigen and diminution or total loss of production of the antibody. 5. Reversion from the R form to the S form, it is possible to have obtained by rapid transfers (15-20 transfers) of the R form variants through a highly nourishing media (3% glucose Sabouraud's broth or agar) at pH 4.0.