Can Raman spectroscopy be used to measure qualitative differences in the organic phase of bones with very different mineral volume fractions?

Abstract

Event Abstract Back to Event Can Raman spectroscopy be used to measure qualitative differences in the organic phase of bones with very different mineral volume fractions? K Buckley1*, P Matousek1, AW Parker1 and AE Goodship2 1 Harwell Oxford, Central Laser Facility, STFC Rutherford Appleton Laboratory, United Kingdom 2 Royal National Orthopaedic Hospital, UCL Institute of Orthopaedics and Musculoskeletal Science, United Kingdom The chemical composition, and hence the mechanical properties, of bone can vary according to its function. The biochemical mechanisms which control this compositional variation are not well understood but we hypothesize that it is guided by the some feature of the collagen chemistry. Raman spectroscopy is an analytical technique which uses the inelastically scattered photons to probe chemical composition. It can be applied to measure relative proportions of hydroxyapatite and collagen in different bony materials, and we hypothesize it can be used to elucidate what determines it. The elucidation of the control mechanism, apart from being interesting from a biochemical point of view, could provide therapeutic targets for the management of bone conditions i.e. enabling the control of the mechanical properties of bone. In this study we inspect the Raman spectra of various functionally adapted bones with very different mineral to collagen ratios. These include bones with relatively large proportions of mineral from the ear (e.g. fin whale tympanic bulla) adapted to be very stiff and bones with relatively large proportions of collagen (e.g. red deer antler). The interesting result is the different bones have differences in the profiles of the Raman spectra of the collagen bands as well as in their intensities. The next step of the research will be to deduce what feature of the collagen chemistry these Raman profile changes represent, and to establish the causal relationship from the spectra to mechanical properties. Keywords: Bones, Bone Research Conference: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society, Cambridge, United Kingdom, 27 Jun - 29 Jun, 2011. Presentation Type: Poster Topic: Abstracts Citation: Buckley K, Matousek P, Parker A and Goodship A (2011). Can Raman spectroscopy be used to measure qualitative differences in the organic phase of bones with very different mineral volume fractions?. Front. Endocrinol. Conference Abstract: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society. doi: 10.3389/conf.fendo.2011.02.00009 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2011; Published Online: 30 Sep 2011. * Correspondence: Mr. K Buckley, Harwell Oxford, Central Laser Facility, STFC Rutherford Appleton Laboratory, United Kingdom, kevin.buckley@stfc.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers K Buckley P Matousek AW Parker AE Goodship Google K Buckley P Matousek AW Parker AE Goodship Google Scholar K Buckley P Matousek AW Parker AE Goodship PubMed K Buckley P Matousek AW Parker AE Goodship Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.