Camelina (Camelina sativa(L.) Crantz) as an alternative oilseed: molecular and ecogeographic analyses

Abstract

Camelina (Camelina sativa (L.) Crantz) is an oilseed known for its potential as a low-input biofuel feedstock and its high levels of beneficial fatty acids. We investigated the role of geographical origin in genetic variation and fatty acid content, expecting to find significant variability among 53 accessions and a link between ecogeography and both origin and key oil traits. Amplified fragment length polymorphism (AFLP) fingerprinting revealed high levels of diversity within the 53 accessions. Even though sampling was relatively biased towards the Russian-Ukrainian area, this region was identified as a genetic diversity hotspot and possible centre of origin for camelina. The accessions were categorized by principal coordinate analysis using molecular marker data, enabling identification of links between geographical distribution and these categories. The influence of geographic location on four canola oil quality measures in camelina was evaluated using a geographic information system. These measures were (1) more than 30% alpha-linolenic acid, (2) less than 3% erucic acid, (3) less than 10% saturated fatty acids, and (4) a ratio of alpha-linolenic to linoleic acid greater than 1. The results clearly confirm that camelina oil quality characteristics are strongly influenced by environmental factors. The unprecedented high genetic diversity in this group of accessions offers an excellent opportunity to investigate valuable genes for successful adaptation of camelina to specific ecogeographical conditions such as drought.