Abstract
The article presents the results of studying the Calpastatin (CAST) gene polymorphism in Tsigai (n = 23) and Merinoland sheep breed (n = 13). The purpose of the research. The possibility of using PCR-RFLP analysis to detect the Calpastatin (CAST) gene polymorphism in Tsigai and Merinoland sheep was the primary objective of this ongoing study. Research methods. Calpastatin (CAST) gene polymorphism was analyzed by PCR-RFLP analysis using the MspI endonuclease restriction. Results. We revealed the diversity of genotypes and allelic variants of the Calpastatin (CAST) gene in Tsigai and Merinoland Breeds. It was found that M allele of the Calpastatin locus is the most common. Frequencies of MM, MN and NN genotypes were found to be 74, 4 and 22 % in Tsigai breed. Among the representatives of Merinoland sheep, the frequencies of MM and MN genotypes were 92 % and 8%, respectively. No animals with NN genotypes were found.
Highlights
It has been known the tenderness depends largely on the amount of connective tissue, myofibrillar protein degradation, and intramuscular fat content
Calpastatin gene polymorphism determination in meat sheepbreeding is a crucial factor among molecular genetic markers [7]
The objective of this study was to identify the possibility of using PCR-RFLP analysis to detect the Calpastatin (CAST) gene polymorphism in Tsigai and Merinoland sheep breeds
Summary
It has been known the tenderness depends largely on the amount of connective tissue, myofibrillar protein degradation, and intramuscular fat content. The tenderness of the meat is largely determined by genetic factors of the livestock itself. One of the genetic componen with an effect on meat tenderness is gene calpastatin [1, 2]. Calpastatin is a regulatory protein, highly specific endogenous μ- and m-calpains inhibitor (calcium-dependent neutral proteinase), which has no effect on other proteases (papain, B and D cathepsins, bromelain, trypsin, chymotrypsin, pepsin, plasmin, etc.). This calpains inhibitor was first identified in muscle. Calpastatin gene polymorphism determination in meat sheepbreeding is a crucial factor among molecular genetic markers [7]
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