Calpains and calpastatin distribution in bovine, porcine and ovine skeletal muscles

Abstract

The concentration of calpain II and calpastatin was determined in various beef, lamb and pork muscles showing very different metabolic and contractile types as assessed by measurement of lactic dehydrogenase (LDH), citrate synthetase (CS) and ATPase activities. The calpain II: calpastatin ratio, which is a good index of the efficiency of this proteolytic system, was also determined. A species comparison revealed that while calpastatin level was lowest in pork, the ratio of calpain II to calpastatin was highest in this species. For both determinations, lamb was intermediate followed by beef. Conversely, the amount of calpain II was very similar in the three species. In beef and pork, calpain II content decreased as muscle ATPase and LDH activities rose; and conversely increased with CS activity; whereas in lamb, the amount of this enzyme was highest in red muscles regardless of their speed of contraction. Except for masseter muscle, a comparable distribution was observed for calpastatin in beef and pork muscles. In lamb, the calplastatin concentration was highest in slow-twitch red muscles, intermediate in fast-twitch red muscles and lowest in fast-twitch white muscles. Variability of the calpain II: calpastatin ratio with muscle ATPase, LDH and CS activities appeared to be both muscle and species dependent. As results for masseter muscle are rather unexpected, especially in beef and lamb, this muscle was considered separately. The present findings are discussed with regard to the conditioning rate of meat from different species and, within one species, from different muscles. It was concluded that the conditioning rate may be correlated positively to calpain II: calpastatin ratio and negatively to calpastatin content. In contrast, no relationship seems to exist between meat ageing rate and calpain concentrations.