Callus induction and plant regeneration from an ancient and forgotten crop plant Vicia ervilia L. (WILLD)

Abstract

Recently ICARDA has recommended the cultivation of annual legume of vetch group especially bitter vetch (Vicia ervilia L.) in fallow lands for right exploitation of pastures and rectifies feed shortage at marginal dry lands areas. To reach this aim a research was carried out in Tabriz university of IRAN. At this research bitter vetch plants and embryo axis were grown and developed under some levels of hormonal concentrations (high and low) on MS medium containing BAP and NAA (0, 0.1, 1, 20 and 40 mg/l). After statistical comparison, hormonal combination of 5 mg/l of BAP with 20 mg/l NAA and without NAA was considered as the best treatment for callus induction and maintenance. Then some callus and embryonic axis were transferred on regeneration medium containing (NAA 0.1 mg/l, BAP 0.2 mg/l), (NAA 2 mg/l + BAP 0.2 mg/l), (NAA 1 mg/l + BAP 0.1 mg/l) and without hormones. Three different hormonal media did not show significant differences for callus size and fresh weights but shooting frequency was high (94%) and the highest number of shoot (6–28 per explant) were obtained and recognized significant difference in comparison to previous experiments which carried out without hormonal shocks.