Abstract
Protoplasts isolated from cell suspension cultures of two caryophyllaceous species, Dianthus caryophyllus L. and Gypsophila paniculata L., were fused using polyethylene glycol and cultured in Murashige and Skoog (1962) (MS) medium containing 1 mg l −1 picloram and 0.7 M sorbitol. Among the calli produced, one showing green coloration and vigorous growth was identified as an intergeneric somatic hybrid based on isozyme analyses for esterase and glutamate dehydrogenase. Hybridity of this callus was also confirmed by Southern hybridization analysis using a rice rDNA probe. This somatic hybrid callus regenerated roots after 2 months of culture on MS medium containg 1 mg l −1 picloram, but shoot regeneration was not observed on any of the media tested.
Published Version
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