Californian worms biomass growth and its zinc accumulation under different concentrations of the metal in the nutrient medium

A V Kharchenko,O I Rozputnii,O I Sobolev,P M Karkach,S V Merzlov,Y O Mashkin

doi:10.32718/nvlvet-a9002

Abstract

The ability of worms to accumulate biotic metals in their body creates the preconditions for obtaining a protein-mineral supplement containing metals in the organic compounds. Experimental studies on the worms reproduction, their mass increase and zinc organic forms accumulation in their body, depending on the content of this metal in the nutrient medium were carried out. Zinc content in the nutrient medium for worms was regulated by adding various doses of this metal salt (ZnSO4 • 7H2O). Worms were grown for 110 days on a nutrient medium with a different content of zinc. It has been established that adding Zinc at a dose of 160 mg/kg of nutrient medium provides increase in the worms number and weight by 1.5–1.9 and 1.55–1.96 times, respectively, as compared to the worms grown on a nutrient medium without zinc addition. The negative impact of zinc at a dose of 640 mg/kg of nutrient medium on the worms mass increase was established. Vermiculture mass was 12.9% lower than the control under high dose of the metal. Additional application of zinc at doses of 40 mg/kg, 80, 160, 320 and 640 mg/kg in growing worms on a nutrient medium provided increase in the content of this element in vermiculture dry mass, respectively, by 20.4%, 31.7, 50.0% or by 2.7 and 3.0 times.

Highlights

IntroductionManure from various species of animals, peat, sawdust, hardwood, straw, cardboard, paper, tree leaves, fruits and vegetables waste products, waste of meatpacking plants, fish production, organic urban wastewater, distillery waste, sugar and brewer production, as well as riboxin, tetracycline and penicillin production mycelium is the nutrient medium for the red Californian worms hybrid (Eisenia fetida) (Melnyk, 1991; Feruchchi, 1992; Pernak, 1995; Sulzberger, 1998).In the non-waste utilization of organic residues, red Californian worms hybrid increases its biomass, which is an adequate protein-vitamin-mineral feed supplement to the diet of farm animals, fish and poultry (Das & Dach, 1989; 1990; Mason et al, 1992; Kostecka & Pączka, 2006).Worms dry matter contain up to 65.0% of protein, up to 11.0% of lipids, of which a large proportion is represented by phospholipids
The number of adult worms increased by 26.5% (Р ≤ 0.01) under vermiculture cultivation on a nutrient medium with an additional 40 mg/kg content of zinc
The worms number and mass increase depends on zinc concentration in the nutrient medium

Summary

Introduction

Manure from various species of animals, peat, sawdust, hardwood, straw, cardboard, paper, tree leaves, fruits and vegetables waste products, waste of meatpacking plants, fish production, organic urban wastewater, distillery waste, sugar and brewer production, as well as riboxin, tetracycline and penicillin production mycelium is the nutrient medium for the red Californian worms hybrid (Eisenia fetida) (Melnyk, 1991; Feruchchi, 1992; Pernak, 1995; Sulzberger, 1998).In the non-waste utilization of organic residues, red Californian worms hybrid increases its biomass, which is an adequate protein-vitamin-mineral feed supplement to the diet of farm animals, fish and poultry (Das & Dach, 1989; 1990; Mason et al, 1992; Kostecka & Pączka, 2006).Worms dry matter contain up to 65.0% of protein, up to 11.0% of lipids, of which a large proportion is represented by phospholipids. Manure from various species of animals, peat, sawdust, hardwood, straw, cardboard, paper, tree leaves, fruits and vegetables waste products, waste of meatpacking plants, fish production, organic urban wastewater, distillery waste, sugar and brewer production, as well as riboxin, tetracycline and penicillin production mycelium is the nutrient medium for the red Californian worms hybrid (Eisenia fetida) (Melnyk, 1991; Feruchchi, 1992; Pernak, 1995; Sulzberger, 1998). In the non-waste utilization of organic residues, red Californian worms hybrid increases its biomass, which is an adequate protein-vitamin-mineral feed supplement to the diet of farm animals, fish and poultry (Das & Dach, 1989; 1990; Mason et al, 1992; Kostecka & Pączka, 2006). Worms dry matter contain up to 65.0% of protein, up to 11.0% of lipids, of which a large proportion is represented by phospholipids. Ointments and tinctures made from worm tissues are used to treat bronchitis, asthma, postpartum weakness, smallpox, jaundice and rheumatism (Kholodova et al, 1992; Kholodova et al, 1994; Hasanuzzaman et al, 2010)

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