Abstract
Summary Peroxidase activity was studied in normal non-organogenic, habituated non-organogenic, and habituated organogenic calli of the same sugarbeet ( Beta vulgaris ) strain. Several subcellular fractions (soluble, membrane, ionic wall and covalent wall fractions) were prepared from these cells and assayed for peroxidase activity. All fractions from habituated non-organogenic cells were found less active than those from normal non-organogenic and habituated organogenic cells. Peroxidase release into the medium by the three types of cells was enhanced by 1 m M calcium chloride in proportion to their level of endogenous peroxidase activity. Calcium ionophores increased the effectiveness of calcium on peroxidase secretion only in habituated organogenic cells. Calcium-mediated peroxidase binding to membranes in vitro was also measured for the three cell lines. It was found that membranes from habituated organogenic cells were the most sensitive to calcium. It is proposed that the calcium-mediated binding of peroxidases to membranes could be related to the secretory process of these enzymes.
Published Version
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