Abstract
Calcium and pyridine nucleotide metabolisin have been studied in rat glomerulosa and luteal cells with microspectrofluorimetry. Large luteal cells (derived of granulosa cells) were obtained from pseudopregnant rats. Cytoplasmic Ca” concentration was measured with fura-2, mitochondrial Ca2’ concentration with rhod-2, the cumulative level of NADH and NADPH was monitored by their autofluorescence. Glomerulosa cells respond to physiological concentrations (1 00-300 pM) of angiotensin I1 with oscillatory cytoplasmic Ca” signal that is precisely followed by similar changes in mitochondrial NAD(P)H level. Physiological and slightly supraphysiological elevation of extracellular K’ concentration (from 4.6 to 8.4 mM, as compared with the control 3. mM) induces sustained increase in cytoplasmic [Ca”] and this signal is followed by sustained increase in mitochondrial NAD(P)H level. Capacitative Ca” influx from the extracellular fluid, induced by depletion of the InsP, sensitive calcium stores, also raises cytoplasmic Ca2+ concentration and mitochondrial NAD(P)H level. The mitochondrial pyridine nucleotide response requires Ca2’. In K’ stimulated cells the inhibition of steroid production with aminoglutethimide decelerates the
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