Abstract
Removal of Ca 2+ from the incubation medium by addition of 2 mM ethylene glycol bis-(β-aminoethyl ether)-N,N′tetraacetic acid (EGTA) leads to at least 75% inhibition of the luteinizing hormone-releasing hormone (LH-RH)-induced accumulation of adenosine 3′5′monophosphate (cyclic AMP) in rat anterior pituitary gland in vitro. This inhibitory effect of EGTA is reversed by the addition of Ca 2+. A half-maximal effect of Ca 2+ on LH-RH-induced cyclic AMP accumulation is observed at 2–5 × 10 −5 M free Ca 2+. The LH-RH induced LH and FSH release is completely dependent upon the presence of Ca 2+ in the incubation medium, a half-maximal effect being measured at 1–2 × 10 −4 M free Ca 2+. The basal release of LH is increased upon Ca 2+ removal.
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