Calcium phosphate adsorption patterns of virulent and avirulent strains of poliovirus

Abstract

The behavior of various strains of all three poliovirus types on calcium phosphate columns was investigated. The average recovery of all strains from such columns was 68%. The highly neurovirulent strains of type 1 poliovirus, Brunhilde and Mahoney, were eluted from calcium phosphate columns at a substantially lower molarity of phosphate buffer than was the attenuated strain LSc,2ab. However, the elution curves obtained with three strains of type 2 poliovirus (that differ sharply in their neuro-virulence), although differing somewhat in shape, all exhibited a peak of eluted virus at 0.2 M phosphate buffer. Three strains of type 3 poliovirus yielded elution patterns that differed significantly one from the other. The highly virulent Saukett strain was eluted in what was essentially a single peak at 0.2 M phosphate, the elution curve of the low neurovirulent strain Leon exhibited three peaks at 0.025, 0.15, and 0.2 M phosphate buffer, and the attenuated strain Leon 12a 1b was eluted maximally from the calcium phosphate column by 0.15 M phosphate buffer.