Cadmium tolerance and co-tolerance in Silene vulgaris (Moench.) Garcke [=S. cucubalus (L.) Wib.

Abstract

This paper is the first to report cadmium tolerance in a dicotyledonous species, Silene vulgaris (Moench.) Garcke. The response to cadmium of five populations originating from one uncontaminated and various heavy-metal contaminated sites was examined under standardized conditions for three weeks. The tolerance index (TI), based on the mean relative growth rate (R), was determined. Populations originating from cadmium-contaminated sites showed a distinct tolerance to cadmium. A population from a site enriched only with copper also exhibited a marked co-tolerance to cadmium. A clear difference in biomass production between the sensitive and tolerant populations was attained at 1 μM cadmium. An optimum biomass production in tolerant populations at a metal concentration higher than in the control, as demonstrated for zinc and copper, could not be established for cadmium. The pattern of cadmium uptake and translocation differed between tolerant and sensitive populations. All tolerant populations accumulated cadmium in the roots and showed some degree of restricted transport to the shoots. The effect of cadmium on the elemental distribution in roots and shoots was population-independent for some elements (copper, zinc, potassium) and population-specific for others (phosphorus, magnesium and sodium). The phenomenon of co-tolerance to cadmium is discussed in relation to possible tolerance mechanisms, especially with regard to metal-binding compounds (metallothioneins, phytochelatins).