Cadmium pollution enhanced ozone damage to winter wheat: biochemical and physiological evidences

Abstract

Combined effects of ozone (O 3) and cadmium (Cd) on growth and physiology of winter wheat ( Triticum aestivum L. cv. JM22) were determined. Wheat plants were grown without or with Cd and exposed to charcoal-filtered air (< 10 ppb O 3) or elevated O 3 (80 ± 5 ppb, 7 hr/day) for 20 days. Results showed that O 3 considerably depressed light saturated net photosynthetic rate (-20%), stomatal conductance (-33%), chlorophyll content (-33%), and total biomass (-29%) without Cd. The corresponding decreases were further enhanced by 45%, 56%, 60% and 59%, respectively with Cd, indicating a synergistic effect of O 3 and Cd on wheat. Ozone significantly increased the activity of superoxide dismutase (46%), catalase (48%) and peroxidase (56%). However, great increases in malondialdehyde (MDA) content (2.55 folds) and intercellular CO 2 concentration (1.13 folds) were noted in O 3+Cd treatment compared to control. Our findings demonstrated that the increased anti-oxidative activities in wheat plants exposed to O 3+Cd might not be enough to overcome the adverse effects of the combination of both pollutants as evidenced by further increase in MDA content, which is an important indicator of lipid peroxidation. Precise prediction model on O 3 damages to crop should be conducted to ensure agricultural production security by considering environmental constraints in an agricultural system in peri-urban regions.