Abstract
Stone cells are sclerenchyma cells formed by deposition of lignin, which is the most significant factor limiting the quality of pears. Ca2+ was known to inhibit stone cells in pear fruits, but the underlying molecular mechanism remains unclear. Our study revealed that exogenous CaCl2 (Ca2+) treatment of “Nanguo” pear (Pyrus ussuriensis) suppressed the synthesis of lignin and stone cell production. We further analysed the transcriptomes using RNA-seq, identified a transcription factor, PuDof2.5, and its targets gene PuPRX42-like (lignin polymerase gene) expression decreased in CaCl2-treated samples, which are involved in suppressing lignin biosynthesis in pear fruit. PuDof2.5 was found to bind directly to the PuPRX42-like promoter and induced its transcription. Taken together, our results revealed that Ca2+ modulated the key lignin biosynthetic transcription factor PuDof2.5 to suppress stone cell production in pear fruits.
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