Abstract
Photoactivatable fluorescent proteins (PAFPs) are molecules that switch to a new fluorescent state in response to specific light activation, and play vital roles in superresolution (SR) imaging. Currently many PAFPs with different colors have been developed; however most of them have limited applications for live cell SR microscopy. Unique PAFPs with specific properties, such as high photo-stability and high signal-to-noise ratio, are needed for different live cell SR techniques. Here we present several novel PAFPs developed recently including mEos3.2, Skylan-S and Skylan-NS. The new fluorescent proteins can be used well for live cell PALM, SOFI and PA NL-SIM imaging for tracking dynamic localizations of tagged proteins with high temporospatial resolution.
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