C-38 Free Communication/Poster - Sports - Lower Extremity

Abstract

Exhaustive exercise caused the physiological and biochemical changes, including short-term decline in the immune system, induction of oxidative stress, and cardiac function impairment. L-Arginine (Arg) could increase nitric oxide synthesis, inhibit inflammation, and exert a good antioxidant property. PURPOSE: To assess the interaction of L-arginine supplementation and exhaustive exercise on hydroxylation modification of serum albumin in rats. METHODS: Forty male Sprague-Dawley rats were randomly assigned to one of the four groups: (1) sedentary control (SC); (2) sedentary control supplemented with 2% L-arginine (Arg+SC); (3) exhaustive exercise (Ex); (4) exhaustive exercise supplemented with 2% L-arginine (Arg + E). The rats were kept in the diets for 30 days. At the end of the study, the rats in control groups were sacrificed, the animals in exhaustive exercise groups were sacrificed immediately after performing a single bout of strenuous exhaustion exercise on a motorized treadmill. After the sacrifice of rats, the blood sample was collected from the abdominal aorta, and centrifuged to obtain the serum. Serum albumin was separated by SDS-PAGE, and collected to assay the post-translational modifications and modification proportion by tandem mass spectrometry. The treatment means were tested for homogeneity using a one-way analysis of variance, and the difference between specific means was tested for significance using Duncan’s multiple-range test. A difference between two means was considered statistically significant when p less than 0.05. RESULTS: There was the same performance of post-translational modification type in 4 groups. Exercise significantly increase hydroxylation on the residue Pro-447 and Asp-451 (p<0.05) but attenuate hydroxylation on Lys-233 and Lys-545 (p<0.05) when compared with sedentary group. The hydroxylation of Lys-545 was significant lower (p<0.05) in Arg+SC group than SC group. Arg + E resulted an addition effect on residue Lys-33 hydroxylation (p<0.01). CONCLUSION: The present study demonstrated that L-Arginine supplementation and exhaustive exercise changed the modification proportion on serum albumin, especially the impact of exhaustive exercise was more than L-Arginine supplementation.