C0 2 Enrichment Delays a Rapid, Drought-Induced Decrease in Rubisco Small Subunit Transcript Abundance

Abstract

Rice (Oryza sativa L. cv. IR-72) was grown in sunlit chambers at 350 and 700 gmol CO2 mol-1 under conditions of continuous flooding (control) or drought which was imposed at panicle initiation, to evaluate the effects of C02 enrichment and soil water deficit on photosynthesis and Rubisco gene expression. Leaf and canopy photosynthetic rates were enhanced by high [CO2] but reduced by drought. High [CO2] and severe drought both reduced rbcS transcript abundance, along with the activity, activation and protein content of Rubisco, but the Km (CO2) was not affected. The transition from moderate to severe drought caused a rapid decline, within 24 h, in the rbcS transcript abundance. High [CO2], however, delayed the adverse effects of severe drought on rbcS transcript abundance and activities of Rubisco, and permitted photosynthesis to continue for an extra day during the drought-stress cycle.