Abstract
Electron microscopic observations of sequential stages of skin carcinogenesis induced by tobacco smoke condensate (SC) and a cyclohexane fraction of tobacco smoke condensate (G) revealed an increase in incidence of intracisternal A particles within the epidermal cells. Tumours induced by SC also contained C-type particles, but these were not seen in G-induced tumours or after irritant or solvent treatment. There was no evidence of an increase in intracisternal A particles after irritant or solvent treatment. A direct relationship between the proliferation of A particles and neoplastic growth of BALB/c mouse epidermis appears likely. The data suggest possible activation of a latent C-type virus by SC.
Highlights
Summary.-Electron microscopic observations of sequential stages of skin carcinogenesis induced by tobacco smoke condensate (SC) and a cyclohexane fraction of tobacco smoke condensate (G) revealed an increase in incidence of intracisternal A particles within the epidermal cells
One hundred and fifty mg
Dose of 200 mg G per week, the first tumour appeared in Week 27, 24/60 animals painted at this dose producing a total of 27 tumours
Summary
Both skin and tumour pieces measuring about 1 mm were fixed in 300 glutaraldehyde in 0-2 M phosphate buffer at 4°C, rinsed in buffer and post-fixed in 1-3300 osmium tetroxide. Sections were cut with a Reichert OMU-3 ultramicrotome, collected on copper grids and stained with lead citrate and uranyl acetate. They were examined on a Philips 301 electron microscope at an accelerating voltage of 80 kV. Sections w%ere prepared at 5 ,um and stained with lhaematoxylin and eosin
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