C-39 Free Communication/Poster - Hypoxia and Altitude Exposure

Abstract

Albumin is the most abundant protein in human plasma, and works as an indicator of tissue function. Albumin also play an important role in which that plasma proteins are the first line to against reactive oxygen species. The functional changes of proteins could be achieved by the post-translational modification. PURPOSE: To assess the interaction of L-arginine supplementation and exhaustive exercise on modification of serum albumin in rats. METHODS: Eight-week-old male SD rats were randomly divided into 4 groups: sedentary control (SC), sedentary supplemented with Arg supplementation (Arg+SC), exhaustive exercise (E), exhaustive exercise supplemented with Arg (Arg + E). Rats in groups with Arg supplement (Arg+SC, Arg+E) received a 2% L-Arg of w/w per day, for 30 days. Rats in exhaustive exercise (E, Arg+E) were treated a single bout exhaustion exercise on a motorized treadmill. Rats were sacrificed immediately after exhaustive exercise in which exhaustive exercise groups (E, Arg+E). After the sacrifice of rats, the blood sample was collected from the abdominal aorta, and centrifuged to obtain the serum. Serum albumin was separated by SDS-PAGE, and collected to assay the post-translational modifications and modification proportion by tandem mass spectrometry. RESULTS: There was the same performance of post-translational modification type in 4 groups. The modifications were identified in 63 amino acid sequences, on 52 amino acid residues, and a total of 14 kinds of modification types. The modification proportion of amino acid residues was analyzed by the one-way ANOVA, there were significant differences (p <0.05) on 15 residues. These modification types included 2’,4’,5’-topaquinone, methylation, nitration, dihydroxylation, hydroxylation, acetylation, hydroxycinnamyl, 2-amino-3-oxo-butanoic acid and S-guanylation. CONCLUSION: The present study demonstrated that L-Arginine supplementation and exhaustive exercise changed the modification proportion on serum albumin, especially the impact of exhaustive exercise was more than L-Arginine supplementation. The results could be as a future research base to understand the follow-up effects on albumin by nutrition intervention and exercise prescription.