Broad-Spectrum Protection Against Several Pathogens by PGPR Mixtures Under Field Conditions in Thailand.

Abstract

Prior greenhouse experiments showed that four mixtures of plant growth-promoting rhizobacteria (PGPR) strains (all Bacillus spp.) elicited induced systemic resistance in several plants against different plant pathogens. Based on these findings, we sought to determine if systemic resistance induced by these PGPR would lead to broad-spectrum protection against several pathogens under field conditions in Thailand. Experiments were conducted during the rainy season (July to October 2001) and winter season (November 2001 to February 2002) on the campus of Naresuan University, Phitsanulok, Thailand. The specific diseases and hosts tested were southern blight of tomato (Lycopersicon esculentum) caused by Sclerotium rolfsii, anthracnose of long cayenne pepper (Capsicum annuum var. acuminatum) caused by Colletotrichum gloeosporioides, and mosaic disease of cucumber (Cucumis sativus) caused by Cucumber mosaic virus (CMV). Results showed that some PGPR mixtures suppressed disease more consistently than the individual PGPR strain IN937a. One PGPR mixture, Bacillus amyloliquefaciens strain IN937a + B. pumilus strain IN937b, significantly protected (P = 0.05) plants against all tested diseases in both seasons. Further, cumulative marketable yields were positively correlated with some treatments.