Broad spectrum of bioactive compounds from halophilic actinomycetes isolated from the Kodiyakadu saltpan of Vedaranyam-India

Abstract

Discovery of new novel drugs are necessitates for treat the bacterial infectious disease. Since, some potent antimicrobial compounds present in soil actinomycetes. Preliminary study was performed to isolate the halophilic actinomycetes from Kodiyakadu salt pan located in Vedaranyam, Nagapattinam District, Tamilnadu, India for promising antibiotic producing actinomycetes against gram positive pathogens- Stapylocoocus aureus, Streptococcus pneumoniae, Vibrio parahaemolyticus, Enterococus faecalis and gram negative pathogens: Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and pseudomonas aeruginosa. Among the six isolates, there had only one of the strain KOA-2 showed good antibacterial activity. Potential strain was confirmed Streptomyces diastaticus accession no (OM060757) by of 16srRNA sequencing molecular profiling technique. Crude extract of KOA-2 strains shows good pathogenic activity of all pathogens and shows high activity in Vibrio parahaemolyticus (32 mm) zone of inhibition. Resazurin assay MIC value represented 0.05 and MBC value 0.312 expressed in mg ml. Crude extract are characterized by GCMS technique. The increased peak value at (16.775) followed by (22.950) retention time were identified as Carbonic acid, propargyl 2- ethylexyl ester and 1-Decane,3,4-dimethyl bioactive compounds are responsible for antibacterial activity against the bacterial pathogens. Finally, crude extracts of KOA-2 strain are characterized by FTIR, UV analysis, and the results are encouraging for biotechnological techniques.