Brevundimonas diminuta isolated from mines polluted soil immobilized cadmium (Cd2+) and zinc (Zn2+) through calcium carbonate precipitation: Microscopic and spectroscopic investigations

Abstract

The toxic metal(loid)s TMs resistant bacterium Brevundimonas diminuta was isolated for the first time from mines polluted soil in Fengxian, China, and assessed for its potential for Cd and Zn precipitation in Cd and Zn co-contaminated aqueous solution at various Cd and Zn levels (20, 40, 80, 160, and 200 mg L−1), pH values (5, 6, 7, 8, and 9), and temperatures (20, 25, 30, and 35 °C). B. diminuta showed a high resistance to both Cd and Zn and was able to precipitate up to 99.2 and 99.7% of dissolved Cd and Zn respectively, at a pH of 7 and temperature of 30 °C. B. diminuta reduced the dissolved concentrations of Cd and Zn below the threshold levels in water. The 3D-EEM analysis revealed the presence of extracellular polymeric substances (EPS) such as tryptophan indicating bacterial growth under Cd/Zn stress. FTIR showed polysaccharides, CO32−, CaCO3, PO43−, and proteins, which may enhance bacterial growth and metal precipitation. SEM-EDS confirmed the leaf-like and granular shape of the biological precipitation and reduction in the percent weight of TMs, which promoted the adhesion/adsorption of Cd2+, Zn2+, and Ca2+. Moreover, XRD analysis confirmed the precipitation of Cd, Zn, and Ca in the form of CdCO3/Cd3(PO4)2, ZnCO3/ZnHPO4/Zn2(OH)PO4/Zn3(PO4)2, and CaCO3/Ca5(PO3)4OH, respectively. These findings indicate that Brevundimonas diminuta can be used for the bioremediation of TMs-contaminated aquatic environments.