Breeding of winter wheat for resistance to leaf rust in the foothill zone of the Central Caucasus

Abstract

The decrease in the efficiency of most Lr-genes is associated with microevolutionary processes within the population and the emergence of new virulent races of the phytopathogen, which are capable of overcoming previously effective resistance genes. The article presents the results of a phytopathological test and marker analysis of the selection material of winter wheat for resistance to the causative agent of brown rust (Puccinia recondita Robex Desm f. sp. tritici.). The object of research was 20 varieties of different ecological and geographical origin. DNA was isolated from the leaves of 10-day-old wheat seedlings. Molecular markers were used for the following genes: Lr9 (SCS5), Lr10 (Fi.2245 / Lr10-6 / r2), Lr19/Sr25 (SCS265), Lr20 / Sr15 (STS638), Lr24 / Sr24 (Sr24 # 12), Lr34 / Sr57 (csLV34), Lr37 / Sr38 / Yr17 / Pch2 / Cre5 (Ventriup / LN2), Lr41 (GDM35), Lr47 (PS10). As a result of molecular screening, it was established that the Lr37 genes were identified in the List 25 cultivar; for the Myth variety-Lr10; for the Eltan variety-Lr10; Markola variety-Lr34; for the Malvina variety-Lr26; the variety Creator-Lr10; for variety DB 1/05-Lr10; the Evklid cultivar has the Lr10 gene; the variety Sumai aut-Lr34; in the Lebidka odes’ka variety-Lr34; Solara variety-Lr34; the variety Zhiva-Lr10, Lr34. When comparing the results of marker analysis with field resistance to leaf rust, a resistant type of reaction to infection (R) reaction (S) was observed in the Markola and Mallyska cultivars; medium susceptible type of reaction (MS)-in cultivars Lebidka odes’ka and Tvorets.