Breed Differences in Pig Liver Esterase (PLE) between Tongcheng (Chinese Local Breed) and Large White Pigs

Qiling Xiao,Lu Yang,Xiliang Wang,Qiongqiong Zhou,Deshi Shi,Yuncai Xiao,Zhongyuan Tian

doi:10.1038/s41598-018-34695-y

Abstract

Human carboxylesterases has been proven to be age and race-related and a sound basis of clinical medication. PLE involve in signal transduction and highly catalyze hydrolysis. Therefore, the expression level of PLE most probably exist age and breed difference and lead to significant differences of pharmacology and physiology. Four age groups of Tongcheng (TC) and Large White (LW) pigs were selected to explore PLE breed and age differences, and it was found that PLE mRNA was most abundant in liver in both breeds. In liver, PLE levels and hydrolytic activities increased with age, and PLE levels (except for 3 month) and the hydrolytic activities were higher in LW than in TC across all age groups. Abundance of PLE isoenzymes was obvious different between breeds and among age groups. The most abundant PLE isoenzyme in LW and TC pigs was PLE-A1 (all age groups) and PLE-B9 (three early age groups) or PLE-G3 (adult groups), respectively. 103 new PLE isoenzymes were found, and 55 high-frequency PLE isoenzymes were accordingly classified into seven categories (A-G). The results of this research provide a necessary basis not only for clinical medication of pigs but also for pig breeding purposes.

Highlights

IntroductionThe C-terminal tetra peptides HXEL bind to the KDEL receptor, locating the carboxylate ester in the endoplasmic reticulum[34]
The highest level of PLE mRNA in both 1-month-old and adult pigs were detected in the liver, which was followed by the kidney, small intestine, skin, fat, and lung; little PLE mRNA was detected in the brain, heart, spleen, muscle, lymph node, and thymus (Fig. 1)
In all the age groups, the PLE level in the liver was the highest, and compared to TC pigs, Large White (LW) pigs showed a higher level of PLE mRNA in the liver(except 3-month-old pigs),kidney(except 1-month-old pigs), and small intestine(except 1-month-old pigs) (Fig. 2)

Summary

Introduction

The C-terminal tetra peptides HXEL bind to the KDEL receptor, locating the carboxylate ester in the endoplasmic reticulum[34]. Based on the above mentioned, it is reasonable to speculate that PLE plays an important role in the pharmacological and physiological effects of drug treatments; PLE expression differences at different ages and in different breeds may lead to pharmacology, toxicology, and physiologic differences. In order to obtain the necessary data for clinically rational drug use and to explore PLE pharmacology and physiology roles, in this study, different age groups of LW pigs and TC pigs were selected to study the expression profile and breed differences in PLE. Differences in total level, abundance, and enzyme activity among PLE isoenzymes from two breeds and four different age groups were systematically studied

Methods

Results

Conclusion