Breast cancer metastasis suppressor-1 inhibits human glioma cells invasion by tissue inhibitor of metalloproteinase-2/matrix metalloproteinase-2 pathway

Abstract

Objective To investigate the effect and mechanism of breast cancer metastasis suppressor-1 (BRMS1) on the human glioma cells invasion.Methods Transfection of the pEGFP-BRMS1 plasmids into the U251 and U87 glioma cells were carried out using Lipofectamine 2000.We studied the role of BRMS1 in glioma cell invasion by matrigel cell invasion assay.We performed gelatin zymography to measure the matrix metalloproteinase (MMP)-2 activities in glioma cells and Western blotting to examine the tissue inhibitorof metalloproteinase-2 (TIMP-2) and MMP-2 proteins expression after BRMS1 restoration.Results Compared to the control plasmids,pEGFP-BRMS1 plasmids could increase the expression of BRMS1 in both glioma cells significantly.In cell invasion assay,BRMS1 inhibits cell invasion ability of U251 and U87 cells in matrigel-coated Boyden chamber by 43％ and 52％,respectively.BRMS1 also suppress the MMP-2 activity.Increasing of BRMS1 expression upregulated TIMP-2 protein expression and inhibited MMP-2 protein expression.Conclusion Our data indicate that BRMS1 may be reduced glioma cells invasion abilities by the imbalance between TIMP-2 and MMP-2. Key words: Breast cancer metastasis suppressor-1; Glioma; Invasion; Tissue inhibitor of metalloproteinase-2 ; Matrix metalloproteinase-2