Regulatory effect of annexin A2 on invasion and migration of human glioma cells

Abstract

Objective To investigate the regulatory effect of annexin A2 (ANXA2) on invasion and migration of human glioma cells. Methods The ANXA2 small interfering RNA (siRNA) and siRNA control were transfected into glioma cells U87 and labeled as ANXA2 siRNA group and siRNA-NC group. The control group was set up at the same time. The expression of ANXA2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting respectively. Cell proliferation was detected by methyl thiazol tetrazolium (MTT). The cell migration and invasive ability was detected by Transwell. Western blotting was used to detect E-cadherin and matrix metalloproteinase (MMP)-2 proteins. Results The levels of ANXA2 mRNA (0.85±0.08, P=0.258) and protein (0.72±0.09, P=0.751) in siRNA-NC group were not statistically differed from those in the control group (for mRNA and protein: 0.87±0.05 and 0.75±0.07). The survival rate [(98.87±11.74)%, P=0.695], the number of invasive cells (40.65±4.85, P=0.410), the number of migrating cells (66.87±7.64, P=0.278) and the levels of E-cadherin (0.70±0.09, P=0.320) and MMP-2 protein (0.29±0.02, P=0.196) in siRNA-NC group showed no statistically significant difference from those in the control group. The survival rate [(52.98±5.61)%, P=0.006], the number of invasive cells (23.22±2.25, P=0.002), the number of migrating cells (41.68±3.87, P=0.002) and the levels of MMP-2 protein (0.11±0.03, P=0.001) in the ANXA2 siRNA group were significantly lower, and the levels of E-cadherin protein (1.14±0.11, P=0.002) were significantly higher than those in the control group (0.72±0.06). Conclusion Interference with ANXA2 can inhibit the proliferation, invasion and migration of glioma cells, reduce the expression level of MMP-2 and promote the expression of E-cadherin. Key words: Glioma; Invasion; Annexin A2; Migration