Brassinosteroid improves seed germination and early development of tomato seedling under phenanthrene stress

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are one of the toxic persistent organic pollutants, have global environmental concern. Seed germination and early seedling development are the initial handicaps for plant establishment in phytoremediation program. Assisted phytoremediation by plant growth regulators may be a potential solution for these problems. Hence, we investigated the effects of seed treatment with various concentrations (0.01, 1.0, 100 nM) of 24-epibrassinolide (EBR) in tomato under graded levels (30, 100, 300 μM) of a three-ring PAH namely phenanthrene (PHE). Delayed and decreased seed germination, reduced length and fresh weight (FW) of shoot and root were observed following 10 days of PHE exposure in a dose dependent manner. Chlorophyll fluorescence study suggested a possible photoinhibition and damage to photosynthetic apparatus under PHE exposure. However, seed treatment with EBR improved seed germination and increased length and FW of shoot and root. In addition, EBR remarkably restored the studied chlorophyll fluorescence parameters towards control levels. Different responses in antioxidant enzymes were observed following PHE exposure, while malondialdehyde (MDA) content was increased in a concentration dependent manner. EBR treatment prior to PHE exposure remarkably increased the activities of antioxidant enzymes over PHE alone, but decreased the MDA contents both in shoot and root of young tomato seedlings. Considering all the studied parameters, seed treatment with 1.0 nM EBR was most effective followed by 100 and 0.01 nM for the improvement of germination and seedling growth under PHE stress in tomato.