Brassica oleracea var. capitata L. Alleviates Indomethacin-Induced Acute Gastric Injury by Enhancing Anti-Inflammatory and Antioxidant Activity

Seong Hwan Ryou,Moon Bong Kim,Il Je Cho,Young Sam Kwon,Beom-Rak Choi,Sae Kwang Ku

doi:10.3390/pr9020372

Seong Hwan Ryou, Moon Bong Kim + Show 4 more

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https://doi.org/10.3390/pr9020372

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Abstract

Brassica oleracea var. capitata L. (white cabbage) is a valuable vegetable with diverse nutraceutical benefit. Present study aimed to investigate the preventive effects of B. oleracea extract (BOE) standardized by vitamin U on indomethacin (IND)-induced acute gastric injury in Sprague-Dawley rats. Pre-administration of three different doses of BOE (12.5–50 mg/kg) for 14 days significantly decreased visible ulcerative lesions in the gastric tissue. In addition, BOE alleviated IND-mediated increase in histological score with inhibiting invaded percentage of lesion and restoring mucosa thickness in peri-ulcerative region. BOE increased the gastric tissue bound to Alcian blue and inhibited the decrease in hexose, sialic acid, and collagen levels by IND, suggesting that BOE protects the gastric tissue through preserving mucus and mucosal integrity. Moreover, BOE pre-administration blocked the reduction of prostaglandin E2 and down-regulated histamine and mRNA expression related to secret gastric acid. Furthermore, BOE mitigated inflammatory responses in the gastric tissue by decreasing activity of myeloperoxidase and expression of nuclear factor-κB-dependent inflammatory genes. BOE also suppressed malondialdehyde with preventing the reduction of glutathione, superoxide dismutase, and catalase in the gastric tissue. Therefore, results from present study suggest that BOE will have a potential for preventing gastric injury.

Highlights

Gastric ulcer affects about 5–10% of the general population during their lifetime [1]. gastric ulcer is caused by imbalance between multiple aggressive and cytoprotective factors, the use of non-steroidal anti-inflammatory drugs (NSAIDs) and infection with Helicobacter pylori are considered as the major risk factors for accelerating hypersecretory acidic environment in the gastric tissue
As the process for discovering novel gastroprotective candidates, we recently reported that pre-administration of B. oleracea extract (BOE) standardized by vitamin U could protect the gastric tissue from acidified ethanol-induced ulcer in mice [20]
Prior to evaluate gastroprotective effect of BOE, we quantified the concentration of vitamin U, a representative gastroprotective compound dissolved in B. oleracea [18], in BOE

Summary

Introduction

Gastric ulcer is caused by imbalance between multiple aggressive and cytoprotective factors, the use of non-steroidal anti-inflammatory drugs (NSAIDs) and infection with Helicobacter pylori are considered as the major risk factors for accelerating hypersecretory acidic environment in the gastric tissue. NSAIDs have been prescribed extensively because of their analgesic, anti-inflammatory and antipyretic activities, but the use of NSAIDs increases relative risk of gastric ulcer by 4.7 times [2]. In order to prevent gastric ulcer caused by NSAIDs, great attention has been paid to natural resource exhibiting anti-inflammatory and antioxidant activities, and several edible plants and those-derived phytochemicals have been suggested as promising alternative candidates [6,7,8,9]

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