BRAF V600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study

Abstract

Gliomas considered the most common primary malignant brain tumors in adults. BRAFV600E mutation occurs more in pediatric gliomas but less frequent in adult gliomas. This study aims to assess the frequency of BRAF mutation in Iraqi patients with gliomas by immunohistochemical study and to correlate its immunoreactivity with some clinicopathological parameters This work did on formalin fixed, paraffin embedded tumor tissue took from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that was manual tissue microarray, in which twenty-four small cores (each measure 2mm) of represented tissue make, and then cut by microtome. Immunohistochemical detection of BRAFV600E antibody did by Dako autostainer link 48. BRAFV600E expressed as cytoplasmic staining in seven (10.60%) cases of glioma. It detected in pleomorphic xanthastrocytoma, oligodendroglioma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic oligoastrocytoma and primary glioblastoma (100%, 9.1% 8.6%, 20.0%, 25% and 6.7%). It had a strong association with pediatric gliomas. From the work concluded that BRAFV600E mutation occurred in low percentage in gliomas especially adult types and significantly express in pediatric gliomas and some rare glial tumors.