Abstract
In membranes of neuroblastoma × glioma hybrid (NG108-15) cells, bradykinin (EC 50 ≅ 5 nM) stimulates GTP hydrolysis by a high-affinity GTPase ( K m ≅ 0.2 μM). The octapeptide, des-Arg 9-bradykinin, was inactive. Stimulation of GTP hydrolysis by bradykinin and an opioid agonist was partially additive. Treatment of NG108-15 cells with pertussis toxin, which inactivates N i, eliminated GTPase stimulation by the opioid agonist but not by bradykinin. The data suggest that bradykinin activates in NG108-15 membranes a guanine nucleotide-binding protein which is not sensitive to pertussis toxin and which may be involved in brady-kinin-induced stimulation of phosphoinositide metabolism in these cells.
Published Version
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