Abstract
This chapter describes the isolation and characteristics of bovine transfer factor, the preparations of antigens from oocysts of E. stiedai, a natural parasite of rabbits and E. bovis, a natural parasite of cattle, which have enabled us to demonstrate and characterize delayed hypersensitivity (DH) by skin testing in these animals Chromatographic fractionation and biochemical characteristics of bovine TF were investigated. The yield of crude TF was about 16 g per calf which enabled us to use the same product in both the biological and biochemical characterization. The TF preparations were analyzed by H2SO4–cysteine reaction for pentoses and quantitated with d-ribose and yeast RNA as standards. The ribose content was 8.8 to 9.8 μg/mg crude TF. The RNA content was 43.81 to 48.1 μg/mg crude TF. Analysis of DNA content by the cysteine reaction using calf thymus DNA as standard gave 10.0 to 16.7 μg/mg crude TF. The actual content of RNA was 27.1 to 33.8 μg/mg crude TF after subtraction of the DNA values.
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