Abstract
Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In the present study, we attempted to isolate and characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was maintained for up to 20 or more passages. We observed a significant increase in the proliferation of boT-MSCs during the 20 consecutive passages under low-glucose Dulbecco’s modified Eagle’s medium culture condition among the three culture conditions. These boT-MSCs presented pluripotency markers (octamer-binding transcription factor 3/4 (Oct3/4) and sex determining region Y-box2 (Sox2)) and cell surface markers, which included CD13, CD29, CD44, CD73, CD90, CD105, CD166, and major histocompatibility complex (MHC) class I (MHC-I) but not CD11b, CD14, CD31, CD34, CD45, CD80, CD86, CD106, CD117, and MHC-II at third passage. Moreover, these boT-MSCs could differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Thus, the present study suggests that the tongue of bovines could be used as a source of bovine MSCs.
Highlights
Mesenchymal stem cells (MSCs) are multipotent cells with the ability to differentiate into several cell lineages, and hold therapeutic implications for cell therapy in field, such as regenerative medicine and reproductive biotechnology
MSCs have been isolated from many species, which includes humans and animals, data are currently limited on isolation from large animals, such as cattle and sheep, using their ruminants
The turnover rate of the mouse lingual epithelium, which is at a rate of 6–7 days, is four- to five-fold higher than that of dorsal skin [2], which suggests the existence of stem cells in the papillae
Summary
Mesenchymal stem cells (MSCs) are multipotent cells with the ability to differentiate into several cell lineages, and hold therapeutic implications for cell therapy in field, such as regenerative medicine and reproductive biotechnology. The development of large animal experimental models, which includes cattle, may provide alternative strategies to investigate MSCs physiology and potential application in human and veterinary regenerative medicine [1]. Lingual dorsal epithelium contains four different kinds of papillae: filiform, fungiform, foliate, and circumvallate papillae. Stratum corneum is observed in filiform papillae, but not in fungiform, foliate, or circumvallate papillae. Taste buds are observed in fungiform, foliate, and circumvallate papillae, but not in filiform papillae. The turnover rate of the mouse lingual epithelium, which is at a rate of 6–7 days, is four- to five-fold higher than that of dorsal skin [2], which suggests the existence of stem cells in the papillae. Hume and Potten [3] revealed that mouse lingual epithelial stem cells are located in the basal layer of the lingual epithelium – similar to other epithelial tissues that employ the 3H-TdR label-retaining
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