Abstract
Intact bovine brain clathrin triskelia, comprising three heavy and three light chains, require either 2 mM calcium or the assistance of protein co-factors for efficient assembly into regular cage structures (Keen, J. H., Willingham, M. C., and Pastan, I. (1979) Cell 16, 303-312). In contrast light chain-free heavy chains assemble readily in the absence of co-factors or calcium. Reconstitution of intact clathrin from heavy and light chains restores the calcium requirement. Our data indicate that light chains impede assembly by creating a kinetic trap rather than by perturbing the affinity of heavy chains for each other. This property suggests a function for light chains as regulatory subunits for clathrin assembly.
Highlights
Intact bovine brain clathrin triskelia, comprising is apparently not required foirn uitro assembly, is connected three heavy and three light chains, require either 2 to the distal segment by a protease-sensitive linker region mM calcium or the assistance of protein co-factors for (11-13)
A coated vesicle-mediated which phosphorylates serinesat positions 11and 13(24).The transport cycle is probably initiated by a n energy-dependent function of light chain phosphorylation remains to be elucinucleation reactionwhich leads to the formatioofna clathrin- dated. coated membrane patch on cythtoeplasmic surfacesof cellular Whether light chains are requirfeodr clathrin assembly has membranes (4)
The to thecontrol of regulatory cytosolic factors that act through structural unit that forms the polygonal lattice consists of the clathrin light chains
Summary
Intact bovine brain clathrin triskelia, comprising is apparently not required foirn uitro assembly, is connected three heavy and three light chains, require either 2 to the distal segment by a protease-sensitive linker region mM calcium or the assistance of protein co-factors for (11-13). The clathrin is thereby chain-free heavy chains from bovine brain tissue induced to polymerize into the characteristpicolygonal lattice, assemble very efficientlyunder standard conditions (isolation which invaginates the membrane and buds into the cytoplabsumffer plus calcium), buwt ill do so even in circumstances as a clathrin-coated vesicle (5). This structure is the substratein which intact clathrin triskelia require additional factors for the uncoating ATPase, whichdissociates clathrin from such as adaptors or assembly proteins (27) to self-associate. The to thecontrol of regulatory cytosolic factors that act through structural unit that forms the polygonal lattice consists of the clathrin light chains
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
