Bovhyaluronidaze azoximer as an antimicrobial alternative in companion animals

A V Nazarova,A I Kozitcyna,V A Guseva,T Sh Kuznetsova,B N Videnin,B S Semenov

doi:10.1088/1755-1315/723/2/022019

Abstract

Antimicrobial resistance formation in various bacteria is a serious biosafety threat. In this regard, the important issue in medical and veterinary fields is not only the rational use of antibacterial drugs, but also the search for ways to significantly reduce the need for antibiotics. Three studies designed to research into these challenges are described. The results indicated that (1) the bacteriuria presences in 62.4 +/- 4.1% of urine samples obtained from companion animals (the microflora count in urine samples is estimated from ‘++’ to ‘Entire Field-of-View’); (2) 16.3 +/- 4.0% of samples from companion animals showed bacteria with low antibiotics sensitivity, and 8.1 +/- 3.0% of samples contained bacteria resistant to 30 antibiotics included in the susceptibility test; (3) the Bovhyaluronidaze azoximer reduce antimicrobial therapy duration statistically significant (p=0.0089). The studies were conducted at the facilities of St. Petersburg State University of Veterinary Medicine and Bars veterinary clinic chain in St. Petersburg, Russia. A randomized clinical trial of Bovhyaluronidaze azoximer effect on course of disease and treatment duration of acute and chronic prostatitis and urocystitis, including complicated by urolithiasis, was conducted in 54 animals. Animals were randomly assigned to intervention (n=34) and control (n=20) groups. The animals of both groups received the antimicrobial therapy. Interventional group patients additionally received Bovhyaluronidaze azoximer in the form of rectal suppositories. The antimicrobial therapy mean duration in intervention group animals took 14.88 +/- 4.98 days, in control group animals — 18.65 +/- 5.40 days. The study findings imply that bacteriuria is widely prevalent in companion animals and antimicrobial resistance is increasing issue in veterinary medicine. Bovhyaluronidaze azoximer contributes to microcirculation restore and increases the bioavailability of antibacterial drugs at the site of infection. This makes its relevant for use in the complex treatment for septic and inflammatory processes. This allows to reduce the antimicrobial course duration (and completely decline antibiotic therapy in some cases) and prevent the formation of chronic inflammation and infection sites.

Highlights

Due to the fast rate of antimicrobial resistance widespread in various bacteria, an important biosafety issue in both medical and veterinary fields is the rational use of antimicrobial agents, and the search for medications that can significantly reduce the need for antibiotics
An important component of antimicrobial resistance problem is the formation of bacterial biofilms
It is established that in most cases in animals with urinary tract diseases antimicrobial therapy should be part of a complex therapy. Errors such as unreasonable prescription of antibiotics for the prevention of bacterial infections, improper selection of dose or administration frequency and course duration can reduce the effectiveness of antimicrobial therapy, as well as lead to a variety of side effects, such as the disease transition into a chronic form and the formation of antimicrobial resistance bacteria strains

Summary

Introduction

Due to the fast rate of antimicrobial resistance widespread in various bacteria, an important biosafety issue in both medical and veterinary fields is the rational use of antimicrobial agents, and the search for medications that can significantly reduce the need for antibiotics. As an alternative to antibiotics, research is being carried out on the possibility of using pharmaceutical preparations based on silver such as Silver Chloride Gel [2] and Argentum Quartz (R) [3], probiotics such as Lactobacillus plantarum [4], Curcumin oile [5] and new types of bandages, for example the Lawsonia Inermisgelatin-starch nano-fibrous bandage [6] These methods are only for topical use and do not have a systemic effect, so they does not act upon the site of chronic infection (which often forms after the biofilm formation), and does not affect the immune system. Treatment of animals with chronic urocystitis, including complicated by urolithiasis, as well as with chronic diseases of the prostatic gland

Experimental

Results and discussion

Сonclusion