Abstract

The present study was conducted in order to reveal the genetic diversity andbottleneck in Anatolian Black Cattle (Bos Taurus). Animal material of the studyconsisted of 75 cattle raised in International Center for Livestock Research andTraining. The bottleneck in the cattle breed studied was checked with 10microsatellites markers, amplified in a multiplex polymerase chain reaction (PCR)were used according to recommendation of FAO (2011). A total of 116 alleles wasobserved from microsatellites studied. Overall value belongs to average number ofalleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho),expected heterozygosity (He), the polymorphic information content (PIC), averageheterozygosity (Ĥ), and FIS, known as the inbreeding coefficient, were 11.60, 5.35,0.80, 0.78, 0.80 and 0.012, respectively. All microsatellite markers except INRA23and ETH3 deviated from Hardy Weinberg equilibrium (HWE). Bottleneck wasanalyzed with Bottleneck software according to three different mutation modelsincluding the infinite allele model (IAM), two-phase mutation model (TPM) andstepwise mutation model (SMM). It can be said that there is not any ultimate risk interms of bottleneck considering L–shaped curve showing normal distributionobtained from the analysis.

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