Boosting β-carotene and storage materials productivities by two-stage mixed and monochromatic exposure stresses on Dunaliella salina

Abstract

Growth and product formation of Dunaliella salina, a potent β-carotene source, were investigated under single and two-stage monochromic and mixed illuminations using two LEDs, each emitting red (R), blue (B), or white (W) light. Targeting cell growth in single-stage, WW, RR, and BB, as well as RB illumination, were compared and mixed RB illumination was found most supportive showing the highest cell growth of 1.81 ± 0.008 g/L. Subsequently, new two-stage illuminations (RB-BB and RB-RR) were designed to investigate growth and bio-product formation using RB illumination similarly in the 1st stage followed by separate BB and RR illuminations within the 2nd stage. RB-BB strategy resulted in enhanced productivities of lipid (7.6 mg/L/day), starch (20 mg/L/day), and β-carotene (0.4 mg/L/day) which were respectively higher by 80, 70, and 81% compared to single-stage control (WW). RB-RR strategy stimulated cell growth while it resulted in decreased productivities of products (other than chlorophyll). The highest biomass level of 2.2 g/L and nitrate removal of 80% were obtained in RB-RR while RB-BB resulted in the lowest values of 1.2 g/L and 48%, respectively. Appropriate selection of illuminations in two-stage strategies, therefore, functions to enhance the productivity of important metabolites or cell growth which can have generic applications in other microalgae. NOVELTY STATEMENT Although the effects of a variety of stressful conditions on microalgae product lines have been investigated so far, the effects of two-stage mixed and monochromatic exposure as a light management strategy have not yet been considered. This strategy was inspired by the fact that cell mass alongside the cell content of a product contributes to product productivity. Accordingly, the growth of Dunaliella salina was first examined under single-stage mixed and monochromatic exposure where mixed red-blue light led to the highest biomass formation. Shifting from mixed to different monochromatic exposures was then examined as a stress factor to stimulate product formation. Higher cell factories obtained under mixed exposure in the 1st stage escalated product productivities within the 2nd stage when exposed to monochromatic light.