Abstract
It is well established that mouse strains exhibit different bone phenotypes, however, there is limited data on intramembranous bone regeneration post‐injury challenge in different mouse strains. Here, we determined if intramembranous bone regeneration differed in four wild‐type inbred mouse strains in response to marrow ablation and if bone geometry (medullary canal size) impacted the bone regeneration response. Mechanical femur ablation was performed in 27‐33 week old females of four mouse strains (FVB/N, C3H/He, C57Bl/6, BALB/C). Animals were euthanized 3 weeks post‐ablation; all bone within the medullary canal of the ablated and intact femurs as well as medullary area at the midshaft of the intact femur was analyzed by µCT. In ablated femurs, mean BV/TV showed clear quantitative differences among the four strains (p = 0.002, 1‐way ANOVA). FVB/N (p = 0.007) and C3H/He (p = 0.002) mice had approximately a 4‐fold increase in BV/TV compared to BALB/C mice. Medullary area varied among the four strains (p < 0.001, 1‐way ANOVA). The BV/TV of the regenerated bone was not correlated with the size of the medullary canal. Our results suggest that genetic differences among mouse strains contribute to the bone regeneration response independent of bone geometry. We identified relatively good (FVB/N and C3H) and poor (BALB/C) bone regeneration mouse strains. This differential bone regenerative response to injury was not affected by bone geometry, specifically medullary canal size.
Published Version
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