Abstract
BackgroundThis study is aimed to determine the efficacy of X-Ray Microtomography (micro-CT) in predicting oxytocin (OT) treatment response in rabbit osteoporosis(OP) model.MethodsSixty-five rabbits were randomly divided into three groups: control group, ovariectomy (OVX) -vehicle and OVX-oxytocin group. The controls underwent sham surgery. OVX-vehicle and OVX-oxytocin groups were subjected to bilateral OVX. The rabbits in OVX-oxytocin group were injected with oxytocin. In the 0th, 4th, 8th, 10th and 12th weeks post OVX operation, bone mineral density (BMD) and bone micro-architectural parameters were measured in three groups.ResultsBone mineral density (BMD), bone volume fraction (BV/TV), Trabecular Number (Tb.N), and Trabecular Thickness (Tb.Th) decreased, while Trabecular Spacing (Tb.Sp) and Structure Model Index (SMI) increased overtime in all the three groups. In OVX-oxytocin group, the bone deterioration tendency is slowing down compared with that of the OVX-vehicle group. The BMD of the OVX-oxytocin group was significantly lower than those in the OVX-vehicle group at 12th week (P = 0.017). BV/TV and Tb.Sp in OVX-oxytocin group changed significantly from 8th week (P = 0.043) and 12th week (P = 0.014), which is earlier than that of BMD and other bone micro-architectural parameters.ConclusionBV/TV and Tb.Sp changed prior to BMD and other bone micro-architectural parameters with oxytocin intervention, which indicate that they are more sensitive markers for predicting early osteoporosis and treatment monitoring when using micro-CT to evaluate osteoporosis rabbit model.
Highlights
This study is aimed to determine the efficacy of X-Ray Microtomography in predicting oxytocin (OT) treatment response in rabbit osteoporosis(OP) model
Our results showed that bone mineral density (BMD) had a consistent descending trend along with bone micro-architectural parameters such as Bone volume fraction (BV/TV), Tb.N, Tb.Th, whereas Tb.Sp and Structure Model Index (SMI) increased overtime in rabbit osteoporosis model
Our main goal is to find which parameter of micro-CT examination is a more sensitive and effective marker as to predict early bone degeneration.The mechanism of how OT mitigate bone deterioration had been explored by magnetic resonance spectroscopy and pathological examination in our previous publications [18]
Summary
This study is aimed to determine the efficacy of X-Ray Microtomography (micro-CT) in predicting oxytocin (OT) treatment response in rabbit osteoporosis(OP) model. Estrogen deprivation of experimental osteoporosis model in animals, such as rats, rabbits, is most commonly used for the postmenopausal osteoporosis study. There are extensive literatures using X-Ray Microtomography (micro-CT) to study mouse/mice bone microarchitectural changes. It can accurately detect BMD and other micro-structure parametersas to assess early bone degeneration in animal OP models. The rat skeletal system is different from human of lacking Haversian system and limited bone remodeling according to the literature [3]. These shortcomings create a problem for using this model to assess some of the newer osteoporosis treatment models. The rabbit model is a lot similar to human skeletal system and more appropriate for use [3, 4]
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