Bone Marrow Mesenchymal Stem Cells (BMSCs)-Derived miR-206 Promotes Breast Cancer Development by Activating Hedgehog Gene Signaling

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are an integral part of the tumor microenvironment and involved in tumor evolution. Our aim is to further illuminate the relationship of exosomes of BMSC origin and breast cancer cells in breast cancer. Differential diagnosis was performed by identifying exosomal miR-206 secreted by BMSCs, and RT-PCR detected miR-206 expression in tumor tissues. Transwell assayed cell function and Target scan analyzed the regulatory relationship between Rab23 and miR-206. Rab23 expression was examined by western-blot after the addition of Rab23 and the effect of Rab23 on hedgehog was further verified. We demonstrated that exosomal miR-206 from BMSCs is expressed in tumor tissues and miR-206 mimics significantly inhibited tumor cell invasion and proliferation. miR-206 targets Rab23 and negatively regulates its expression. Further results showed that the addition of Rab23 could activate hedgehog signaling and promote the development of breast cancer. In conclusion, our study reveals that BMSC-derived miR-206 activates hedgehog gene signaling and promotes the breast carcinogenesis development by regulating Rab23 expression.