Abstract
Objective To investigate the feasibility of bone marrow derived mesenchymal stem cell (BMSC) transplantation in the treatment of denervated intrinsic paw muscle atrophy. Methods Twelve adult male Sprague-Dawley rats (24 sides) were randomly divided into ulnar nerve transection group, stem cell transplantation group, culture medium injection group and normal control group. GFP-transfected BMSCs cultured in vitro were injected into the paw muscles of rats in the stem cell transplantation group. After 1 month, intrinsic paw muscles were tested by histology, immunofluorescence and PCR. Results The muscle wet weight and muscle fiber cross-sectional area of stem cell transplantation group were 66% and 63% of normal control group, respectively, and twice as much as that of ulnar nerve transection and culture medium injection groups (P<0.05). GFP-positive mesenchymal stem cells still could be detected at injection site. The expression of VEGF, HGF, CNTF, bFGF in stem cell transplantation group were higher than that in ulnar nerve transection and culture medium injection groups, and the apoptotic index (apoptotic cells/total cells) in stem cell transplantation group was smaller (P<0.05). Conclusion BMSCs can effectively delay the denervated intrinsic paw muscular atrophy. BMSCs may have the capbility of secreting neurotrophic factor and reducing muscle tissue apoptosis. Key words: Mesenchymal stem cell transplantation; Muscular atrophy; Intrinsic paw muscles; Animal experimentation
Published Version
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